Levels of Pathogenic Th17 and Th22 Cells in Patients with Rheumatoid Arthritis

Figure 2

Levels of Th17 and Th22 lymphocytes in patients with RA. Blood samples from 30 patients with rheumatoid arthritis (RA) and 16 healthy controls (Control) were obtained and PBMC were isolated, stained, and analyzed by multiparametric flow cytometry, as stated in Materials and Methods and shown in Figure 1. (a) Percentages of CD4⁺IL-17⁺ cells, referred to total lymphocytes. (b) Percentages of pathogenic Th17 (pTh17) cells referred to total lymphocytes. (c) Absolute numbers of pTh17 cells/ml. (d) Percentage of nonpathogenic Th17 (npTh17) cells, referred to total lymphocytes. (e) Percent of Th22 cells (CD4⁺CXCR3^+/-IL-17^-IL-22⁺), referred to total lymphocytes. (f) Absolute numbers of Th22 lymphocytes/ml. Horizontal lines correspond to the median and - interquartile range. , (Mann–Whitney test).

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