Endothelial Cells Potentially Participate in the Metastasis of Triple-Negative Breast Cancer

Figure 2

scRNA-seq analysis showed three subclusters in breast-associated biopsies. (a) UMAP plot showed integration of endothelial cells isolated from normal breast and TNBC tissues. Three subclusters were identified based on genetic profiles. (b) Violin plots showed the expression pattern of three feature genes ATP1B3, HSPA1B, and KRT7. (c) Immunofluorescent staining of 3 endothelial cell subpopulations: EC (ATP1B3), EC (HSPA1B), and EC (KRT7). Endothelial cells were labelled with CD31. (d) Heatmap showed expression pattern of top 20 genes from each endothelial cell subclusters. (e) Dot plot revealed that each endothelial cell subcluster possesses distinct biological features.

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