Semaphorin-3 Promotes Specific Immunotherapy Effects on Experimental Food Allergy

<div>sema3A modulates Pak1 and RAS activities in sensitized mast cells. BMMCs were sensitized by incubating with anti-OVA-IgE (5 <i>μ</i>g/ml) overnight with or without the presence of sema3A at indicated concentrations. Cells were analyzed via RT-qPCR and RAS-specific ELISA. (a) mRNA levels of Pak1 in BMMCs. (b, c) Immunoblots show Pak1 protein levels in BMMCs. Curves show integrated density of immunoblots of Pak1. (d–f) Curves show levels of RAS.GTP (d), RAS.GDP (e), and RAS activation (f) in BMMCs. 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The data represent 3 independent experiments.</div>

Journal of Immunology Research

fig4

Figure 4

Figure 4: Semaphorin-3 Promotes Specific Immunotherapy Effects on Experimental Food Allergy 