Optimized Protocol for the Isolation of Extracellular Vesicles from the Parasitic Worm <i>Schistosoma mansoni</i> with Improved Purity, Concentration, and Yield

<div>Small iodixanol gradient separates adult worm EVs from non-EV contaminants. Adult worm EV-enriched UC pellets were overlaid with a small (1.7 ml) iodixanol density gradient and centrifuged for two hours. (a) The gradient after centrifugation shows hemozoin (black arrow) in the bottom of the tube. (b) Cryo-EM analysis of washed iodixanol gradient fractions with density 1.07-1.21 g/ml shows that EV-enriched fractions of the small gradients are devoid of hemozoin crystals. Scale bars are 500 nm.</div>

Journal of Immunology Research

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Figure 4: Optimized Protocol for the Isolation of Extracellular Vesicles from the Parasitic Worm <i>Schistosoma mansoni</i> with Improved Purity, Concentration, and Yield 

Figure 4 | Optimized Protocol for the Isolation of Extracellular Vesicles from the Parasitic Worm Schistosoma mansoni with Improved Purity, Concentration, and Yield 