Optimized Protocol for the Isolation of Extracellular Vesicles from the Parasitic Worm Schistosoma mansoni with Improved Purity, Concentration, and Yield

Table 1

Iodixanol gradient volumes and centrifugation details.

(a)

	Rotor type	Gradient build (iodixanol)
		EV sample (μL)	60% (μL)	40% (μL)	30% (μL)	10% (μL)
Large	SW 41 Ti	70	3,200	1,600	1,600	5,500
Medium	SW 55 Ti	70	1,100	550	550	1,980
Small	TLS-55	70	440	220	220	792

(b)

Rotor type	Rpm	Average g-force	k-Factor	Centrifugation time (h)	Total gradient volume (μL)	Fraction volume (μL) (12×)
SW 41 Ti	37,000	169,044	152	>16	11,970	997
SW 55 Ti	42,000	169,639	82	>15	4,250	354
TLS-55	50,000	166,180	60	2	1,742	145