A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641

Figure 3

Selection of the optimal SP concentration and treatment time. (a–d) The optimal SP concentration was selected after testing concentrations of 0, 10, 25, 50, 100, and 150 nmol/L. (a) NK-1R gene expression at each SP concentration. (b–d) The levels of NK-1R, NF-κB p65, AKT, and p-AKT protein expression at each concentration of SP. (e–h) The optimal SP treatment time was selected after incubation for 0 h, 12 h, 24 h, 36 h, and 48 h, respectively. (e) NK-1R gene expression at each SP treatment time. (e–h) The levels of NK-1R, NF-κB p65, AKT, and p-AKT protein expression at each SP treatment time. , vs. 0 nmol/L group or 0 h group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)