MZF1 Transcriptionally Activated MicroRNA-328-3p Suppresses the Malignancy of Stomach Adenocarcinoma via Inhibiting CD44

Figure 3

miR-328-3p negatively modulated CD44 expression. (a) The GEPIA2 and miRTarBase databases were used to identify overlapped genes. (b) The top 10 bottleneck proteins and top 10 hub proteins were selected by utilization of bottleneck and degree algorithm, respectively. (c) The association between CD44 and the prognosis of STAD patients. (d) The CD44 coexpressed genes were predicted to be enriched in 7 GO terms. The -axis represented the gene ratio, and the intensities of different colors represented the adjusted value. (e) The binding sites between miR-328-3p and CD44 predicted by TarBase database were shown. Dual-luciferase reporter assay was employed to evaluate their interaction. (f) RIP assay was also performed to verify the relationship between miR-328-3p and CD44. (g, h). qRT-PCR was utilized to detect the expression level of CD44 in (g) miR-328-3p-downregulated AGS cells and (h) miR-328-3p-overexpressed SNU16 cells. All experiments were performed in triplicate. , .

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