Methane Alleviates Lung Injury through the IL-10 Pathway by Increasing T Regulatory Cells in a Mouse Asthma Model

Figure 2

Methane protects mice from OVA-induced lung inflammation as assessed by EOS, IgE, and protein and mRNA expression of asthma-related cytokines. The representative flow cytometry plot indicates the EOS cell percentage in BALF (a) and these data were visualized (b). IBALF IgE (c) and IL-4 (d) levels, as well as serum IL-5 and IL-10 concentrations, were measured and analyzed by ELISA (e). In the transcriptional analysis, mRNA levels of IL-4, IL-5, and IL-10 in the lungs were evaluated and analyzed by quantitative reverse transcription PCR (f). The data are presented in the form of the ( per group). and denote and , respectively, versus control group; ## denote , respectively, versus asthma group.

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