Partial Reconstruction of Uterus Cervix in Rat by Decellularized Human Uterine Cervical Scaffold Combined with Adipose-Derived Stem Cells (ADSCs)

Figure 2

Evaluation of decellularized human cervix. Macroscopic results: the whitening of the tissue was considered as a visual sign of decellularization (a). Representative microscopic images of native human cervical tissue and decellularized scaffolds from SDS method (b–d). Cellular components were stained by H&E (b). ECM components were stained with Masson’s trichrome staining (c) and Alcian blue staining (d). Scale bars represent 100 μm for H&E and 200 μm for others. DAPI staining in native tissue (E1), decellularized scaffold from DOC method (E2), and SDS method (E3). Cell nuclei in the native tissue showed a typical round-shape based on DAPI staining. While fluorescence from two methods decreased significantly, DNA was isolated and quantified from native tissue and decellularized scaffolds from two methods (f). Data are shown as . indicates statistically significant difference between native tissue and decellularized scaffold from SDS method. IHC staining ECM components including elastin, collagen IV, and fibronectin in native tissue and decellularized scaffold from SDS method are shown (g-i). Scale bars represent 50 μm. No significant change was shown in elastin, collagen IV, and fibronectin after DC (j).