Partial Reconstruction of Uterus Cervix in Rat by Decellularized Human Uterine Cervical Scaffold Combined with Adipose-Derived Stem Cells (ADSCs)

Figure 5

Histological evaluation of reconstruction of partial uterine cervical defect in rat. Histological images of cross-sections of rat uterine cervix from the subtotal excision-only group, decellularized scaffold group, and recellularized scaffold group were shown at 7 days (A1-H1) and 28 days (A2-H2) postsurgery. (a–d) are for H&E staining, and (e–h) are for Masson’s trichrome staining. Black arrows indicate the surgical sites of each group. Scale bars represent 500 μm. Statistical analysis of the thickness of cervical surgical site (i) finds that the cervical surgical site in both scaffold groups was thicker than the subtotal excision-only group and was similar to the normal control group. Masson staining illustrated collagen fibers as blue fibers and was used to detect cervical fibrosis (j). The proportion of fibrotic area of the recellularized scaffold group and normal control group was lower than that of the subtotal excision-only group. There were no significant differences in fibrosis between 7 days and 28 days after surgery in all groups. Data are presented as . .