Partial Reconstruction of Uterus Cervix in Rat by Decellularized Human Uterine Cervical Scaffold Combined with Adipose-Derived Stem Cells (ADSCs)

Figure 8

Cell tracing in vivo results. ADSCs were labeled with DiO (green) (a, b, 10x). Three days after RC with DiO-labeled ADSCs, microscopic images (c) and confocal images showed diffuse green fluorescence throughout the recellularized scaffold. By serial confocal imaging of the scaffold, herein showed fluorescent image of one random transverse section of the scaffold (d) and confocal 3D images (e). Scaffold with DiO-labeled ADSCs was implanted to the rat model, and microphotographs of cross-sections of the rat uterine cervix were taken 3 days after surgery. Merge (f), DAPI (h), and DiO (g) images of fluorescence signals are shown, respectively. Nuclei of DiO-labeled transplanted donor cells and host cells were all stained blue with DAPI and distributed in all parts of the tissue, while green fluorescence with DiO of donor cells only existed in the implant site. Yellow dashed lines represent the border between graft and adjacent original tissue. Scale bars represent 50 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)