Heterogeneity in NK Cell Subpopulations May Be Involved in Kidney Cancer Metastasis

Figure 2

scRNA-seq analysis showing three natural killer (NK) cell subpopulations. (a) UMAP plot showing the clustering in normal kidney cells and clear cell renal cell carcinoma (ccRCC). Three subpopulations were identified based on genetic mapping. (b) Violin plot showing the preferential expressions of the signature genes GZMH, EGR1, and CAPG in three NK cell subpopulations, respectively. (c) Flow cytometric validation of NK subpopulations. NK cells were identified as CD3-CD16-CD56+ cells and were subdivided by counterstaining of antibodies against EGR1, GZMH, and CAPG. (d) Heat map showing the expression patterns of the top 20 genes in each NK cell subpopulation.

(a)

(b)

(c)

(d)