Research Article

LINC01094/SPI1/CCL7 Axis Promotes Macrophage Accumulation in Lung Adenocarcinoma and Tumor Cell Dissemination

Figure 3

CCL7 knockdown suppresses chemotaxis and M2 skewing of macrophages. (a) A sketch map for the Transwell coculture system of PMA-treated THP-1 cells and LUAD cells (A549 and H125); (b) number of migrated THP-1 cells examined by crystal violet staining; (c) expression of M1 cytokines (IL-6, IL-1β, and TNF-α) and M2 cytokines (IL-10 and TGF-β) in the culture medium determined by ELISA kits; mRNA (d) and protein (e) levels of CD86 and CD206 in cocultured THP-1 cells examined by qPCR and WB analyses; (f) staining intensity of CD86 (red) and CD206 (green) in THP-1 cells determined by immunofluorescence staining. . Data are presented as the . .
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