Research Article

LINC01094/SPI1/CCL7 Axis Promotes Macrophage Accumulation in Lung Adenocarcinoma and Tumor Cell Dissemination

Figure 5

SPI1 promotes CCL7 transcription. (a) Transcription factors that can bind to CCL7 promoter with an over 0.5 correlation coefficient in TCGA-LUAD analyzed using the R TFBS package; (b) binding sites between SPI1 and CCL7 promoter; (c) conservative binding sequence of SPI1; (d) a sketch map for the fragmentation of CCL7 promoter sites using the Crispr-Cas9 system; mRNA (e) and protein (f) levels of CCL7 in A549 cells after knockout of A, B, and C fragments alone or simultaneously; (g) CCL7 promoter fragments enriched by anti-SPI1 examined by the ChIP-qPCR assay; (h) construction of the pGL4-Luc luciferase vector containing the CCL7 promoter sequence for the examination of SPI1-CCL7 binding by luciferase assay. . Data are presented as the . .
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