Characterization of the Tumor Microenvironment in Osteosarcoma Identifies Prognostic- and Immunotherapy-Relevant Gene Signatures

Figure 4

Construction of the TMEscore gene signature. (a) The UpSet plot compared high- and low-expression groups as determined using ESTIMATE and ssGSEA and identified 359 interaction genes. (b) CD4+ TMA was differentially expressed in the high versus low immune score groups. (c) CD4+ TMA affects prognosis. (d–g) Venn plots showing the number of interaction genes that are closely related to CD4+ TMA and univariate Cox regression analysis. These genes were subjected to LASSO analysis. -axis: -log (lambda), -axis: LASSO coefficients. LASSO analysis identified 7 genes which were subjected to follow-up analyses. (h) Analysis of correlation between the 7 genes and immune cells. (i) Heatmap visualization of the relationship between the 7 genes and clinical features.