Characterization of the Tumor Microenvironment in Osteosarcoma Identifies Prognostic- and Immunotherapy-Relevant Gene Signatures

Figure 9

The role of EVI2B in OS. (a–c) Associations between EVI2B levels and survival outcomes in TCGA-OS, GSE21257, and Zhujiang cohorts. (d) KM survival curves for CD4 in the Zhujiang cohort. (e) Correlation between the expression of EVI2B and CD4 in the Zhujiang cohort. (f) The Transwell assay showed that EVI2B overexpression markedly suppressed osteosarcoma cell invasion and migration. (g) The plate colony formation assay was performed to investigate the effects of EVI2B on cell proliferation. (h) Effects of EVI2B on apoptosis were evaluated by flow cytometry. (i) Images of the subcutaneous tumor.

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