Targeting Ferroptosis Attenuates Inflammation, Fibrosis, and Mast Cell Activation in Chronic Prostatitis

Figure 5

DFO and EDA alleviated the inflammatory responses in EAP model. (a) Representative HE-stained images of prostate tissues. (b) Inflammation grades for prostate samples were scored on the basis of HE sections (×400 magnification). The columns represent five random spots of one rat, while the rows denote six animals involved in each group. (c) The mRNA expression of proinflammatory cytokines (TNF-α, IL-1β, and IFN-γ) and chemokines (CCL2, CCL3) relative to β-actin was performed by the RT-PCR method. (d) The protein levels of immunological mediators (COX-2 and IL-2) and cytokines (IL-6, VCAM-1, and BDNF) were performed using the western blot method, and the relative quantitative results are shown. Data was presented as . versus the control group; versus the control group; versus the control group; versus the EAP group; versus the EAP group; versus the EAP group.

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