The Proinflammatory Cytokines IL-18, IL-21, and IFN-γ Differentially Regulate Liver Inflammation and Anti-Mitochondrial Antibody Level in a Murine Model of Primary Biliary Cholangitis

Figure 5

Autoimmune cholangitis in IFN-γ^-/-p40^-/-IL-2Ra^-/- mice. (a) Serum AMA levels in IFN-γ^-/-p40^-/-IL-2Ra^-/- () and p40^-/-IL-2Ra^-/- () mice. (b) Representative H&E of the liver at 100x and 400x magnification. (c) Liver pathological score of IFN-γ^-/-p40^-/-IL-2Ra^-/- () and p40^-/-IL-2Ra^-/- () mice. (d) Densities of liver MNCs in IFN-γ^-/-p40^-/-IL-2Ra^-/- () and p40^-/-IL-2Ra^-/- () mice. (e) Numbers of T cells in the liver from IFN-γ^-/-p40^-/-IL-2Ra^-/- () and p40^-/-IL-2Ra^-/- () mice. (f) Numbers of CD4⁺ and CD8⁺ T cells in the liver from IFN-γ^-/-p40^-/-IL-2Ra^-/- () and p40^-/-IL-2Ra^-/- () mice. (g) Percentages of CD8⁺ T central memory (Tcm) and effector memory (Tem) cells from IFN-γ^-/-p40^-/-IL-2Ra^-/- () and p40^-/-IL-2Ra^-/- () mice. (h) Quantitative real-time PCR analysis for mRNA levels of Csf1, Gzmb, and Prf1 in IFN-γ^-/-p40^-/-IL-2Ra^-/- () and p40^-/-IL-2Ra^-/- () mice. .

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