Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen

Figure 3

Effect of the S2-Ag85A vaccine on promoting dsDNA and RNA transmission pathways in IEC and DC cells. RT-PCR detection of (a) IEC cell sensor transduction molecules cGAS (), cGAMP, TBK1, NF-κB, IRAK4, TRAF6, MYD88, and AIM2; (b) DC cell sensor transduction molecules cGAS (), cGAMP, TBK1 (), NF-κB, IRAK4, TRAF6, MYD88, and AIM2. Q-PCR for (c) IEC cell sensor molecules RIG-I, RNA Pol III, and AP1; (d) DC cell sensor molecules RIG-I, RNA Pol III, and AP1. (e) Western blot for AIM2, STING, IRF3, IRF7, and DAI.

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