Research Article
miR-199a-5p Relieves Obstructive Sleep Apnea Syndrome-Related Hypertension by Targeting HIF-1α
Figure 5
miR-199a-5p targeted HIF-1α. mRNA expression of (a) miR-199a-5p and (b) HIF-1α in A7r5 cells under normoxia and hypoxia (1% O2) by qRT-PCR. (c) Protein expression of HIF-1α in A7r5 cells by western blot and (d) quantification. (e) Luciferase reporter assay to validate the putative (f) miR-199a-5p-binding site in the 3-UTR of HIF-1α. WT: wild type; Mut: mutant; NC: negative control. , .
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