miR-199a-5p Relieves Obstructive Sleep Apnea Syndrome-Related Hypertension by Targeting HIF-1α

Figure 6

miR-199a-5p/HIF-1α inhibited proliferation of vascular smooth muscle cells under hypoxia. mRNA expression of (a) miR-199a-5p and (b) HIF-1α in A7r5 cells under normoxia and hypoxia (1% O₂) by qRT-PCR. (c) Protein expression of HIF-1α in A7r5 cells by western blot and (d) quantification. (e) BrdU (red) incorporation in A7r5 cells transfected with the miR-199a-5p mimic, miR-199a-5p inhibitor, and corresponding controls under hypoxia (1% O₂). . (f) Quantification of BrdU-positive cell number. (g) Colony formation assay in A7r5 cells transfected with the miR-199a-5p mimic, miR-199a-5p inhibitor, and corresponding controls under hypoxia (1% O₂). . (h) Quantification of relative number of colonies. NC: negative control. , , and .

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