Methylated RNA Immunoprecipitation Sequencing Reveals the m⁶A Landscape in Oral Squamous Cell Carcinoma

Figure 1

MeRIP-Sep and peak calling analysis. (a) As shown in the flowchart, each group comprised two types of sample RNAs, that is, immunoprecipitation (IP) samples and input control samples, which were fragmented into ~100 nucleotides and immunoprecipitated with m⁶A-specific antibodies. (b) Following the library construction, high-throughput sequencing, and bioinformatics analysis, the location of m⁶A in the whole transcriptome could be provided. Peak calling referred to the use of certain statistical methods to detect the area (called peak) where the reads were remarkably enriched in RNA, as a candidate for m⁶A modification sites. After the peak was obtained, peak sequence motif and peak annotation analyses were performed in the peak to further explore the direction of interest.