Methylated RNA Immunoprecipitation Sequencing Reveals the m⁶A Landscape in Oral Squamous Cell Carcinoma

Figure 2

M⁶A-specific peak sequence motif analysis. In genetics, a sequence motif is a widespread nucleotide or amino acid sequence pattern. In the apparent data analysis, a specific base sequence with a high affinity to certain proteins was generally defined as a motif. M⁶A-specific motif meant a specific base sequence RNA which was modified by m⁶A. The name of the motif was created using the IUPAC codes for nucleotides which had dissimilar letters to express the 15 probable assemblies. The name itself was an expression of the motif although the position weight matrix was not diametrically synonymous as it was produced from the sites that were found to match the letters given in the name. The -value was the enrichment value multiplied by the number of candidate motifs tested. The enrichment value was calculated using Fisher’s exact test for the enrichment of the motif in the positive sequences. The counts used in Fisher’s exact test were made after the erasing of the sites that matched the previously found motifs. Unerased -value was the -value of the motif calculated without erasing the sites of the previously found motifs.