Methylated RNA Immunoprecipitation Sequencing Reveals the m⁶A Landscape in Oral Squamous Cell Carcinoma

Figure 4

Peak frequency distribution in the transcript area. (a) In theory, the m⁶A peak of mRNAs should be enriched near the 3UTR. According to the distribution of peaks in the transcript area, the data characteristics can be understood more intuitively. The R software package Guitar was utilized to calculate the frequency of peaks falling on each site in the mRNA and lncRNA transcript regions for each sample, after which, the frequency distribution map was drawn. The abscissa stood for the 5UTR, CDS, and 3UTR regions on the mRNA transcript and the entire interval of lncRNA, and the ordinate indicated the frequency of peak falling on these regions. (b) The m⁶A sites were analyzed by the Integrative Genomics Viewer (IGV) tool.

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