Methylated RNA Immunoprecipitation Sequencing Reveals the m⁶A Landscape in Oral Squamous Cell Carcinoma

Figure 5

Functional area peak distribution. Metagene profile demonstrated the m⁶A allocation in 5UTR, CDS, stop codon, and 3UTR. After the calculation of the peak distribution in the functional area covered by the peak annotation analysis results, the R software package ChIPseeker was applied to plot the statistical results into a histogram where all samples were compared together and plot each sample into a pie chart for separate viewing. In the histogram, the ordinate represented the sample, and the abscissa stood for the ratio of peaks in each functional area. The pie chart exhibited the exact percentage of peaks in each functional area. 5UTR: the ratio of peaks falling within the 5UTR; 3UTR: the ratio of peaks falling within the 3UTR; 1st exon: the ratio of peaks falling within the first exon of the transcript; other exon: the ratio of peaks falling within other exons except for the first exon of the transcript; 1st intron: the ratio of peaks falling within the first intron of the transcript; other intron: the ratio of peaks falling within other introns except for the first intron of the transcript; distal intergenic: the ratio of peaks falling in the intergenic region.