C/EBPα-Mediated Transcriptional Activation of PIK3C2A Regulates Autophagy, Matrix Metalloproteinase Expression, and Phenotypic of Vascular Smooth Muscle Cells in Aortic Dissection

Figure 3

C/EBPα knockdown and overexpression in aortic VSMCs. C/EBPα was knocked down using three independent siRNAs in aortic VSMCs, and knockdown was confirmed at both mRNA level by RT-qPCR (a) and protein level by Western blot (b). To overexpress C/EBPα in aortic VSMCs, C/EBPα cloned in pcDNA3.1 (pcC/EBPα) was transfected and confirmed at both mRNA level by RT-qPCR (c) and protein level by Western blot (d). Experiments done in triplicate. vs. control.

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