Impact of Notch3 Activation on Aortic Aneurysm Development in Marfan Syndrome

Figure 1

Genes were differentially expressed in the aorta of WT and Fbn1^mgR/mgR (mgR) mice including Notch3, Fbn1, and α-actin. (a) Heatmap of the differentially expressed genes in the aorta of WT and mgR mice at PD28 (/group) was generated using data from RNA-Seq analysis. (b) Notch3 mRNA expression in the aorta of WT () and mgR () mice at PD28 was analyzed by real-time PCR. The bar graph (b) shows relative expression of Notch3 and 18 s rRNA. Immunohistochemical staining of Notch3 in the aortic sections from (c) WT and (d) mgR mice at PD28 (–6/group), respectively. Positive staining is shown in brown (DAB). Notch3-positive cells were quantitated by Definiens Tissue Studio software. Brown chromogen intensity is shown in (e) bar graph (–6/group). compared to WT controls; Student’s -test.