Impact of Notch3 Activation on Aortic Aneurysm Development in Marfan Syndrome

Figure 2

Notch3 activation was increased in the aorta of Marfan patients and Fbn1^mgR/mgR (mgR) mice. Western blot analysis of active Notch3 (N3ICD) levels was performed on aortic protein from WT and mgR mice at PD28 (a) (/group) and Marfan patients and normal controls (/group) (c). The bar graphs show relative N3ICD levels in mouse (b) and human aortic tissue (d), respectively. compared to WT controls; ^† compared to normal controls; Student’s -test. Immunohistochemical staining of human Notch3 in the aortic sections from normal control (e) and Marfan patients (f) (/group). Positive staining is shown in brown (DAB). Notch3-positive cells were counted (cells/high power field, 40x). (g) The values reflect the . ^† compared to normal control; Student’s -test.

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