The BRD4 Inhibitor dBET57 Exerts Anticancer Effects by Targeting Superenhancer-Related Genes in Neuroblastoma

Figure 1

dBET57 impaired the proliferation of NB cells. (a) NB cells and 4 normal cells were treated with gradient concentration of dBET57 for 72 h to analyze the cell viability curve and calculate the value of semi-inhibitory concentration. (b) Morphological changes and cell viability curves (with time as reference) of NB cells treated with dBET57 gradient concentration for 48 h. Scale bars: 100 μm. (c) Colony assay was used to compare the cloning ability of NB cells treated with dBET57 for 48 h. Scale bars: 4 mm. (d) EdU experiment proved the proliferation ability of NB cells incubated with dBET57 for 48 h. Scale bars: 100 μm. The experimental groups (different concentration gradients of dBET57) were compared with the control group (0 nM). , , and ; ns: not significant.

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