The BRD4 Inhibitor dBET57 Exerts Anticancer Effects by Targeting Superenhancer-Related Genes in Neuroblastoma

<div>dBET57 induced apoptosis, inhibit normal cell cycle and migration ability of NB cells. NB cells were treated with different concentration gradients of dBET57 for 48 h. (a) Flow cytometry and Annexin V/PI staining were used to analyze the degree of apoptosis of NB cells. (b) Flow cytometry and PI staining were used to analyze the cell cycle distribution of NB cells. (c) Western blotting showed the protein level expression of Cyclin D1, CDK9, Bcl-2, and cleaved PARP in NB cells. (d) Adhesion assay to evaluate the adhesion ability of NB cells. Scale bars: 200 <i>μ</i>m. (e) Scratch experiment evaluate the migration ability of NB cells. Scale bars: 100 <i>μ</i>m. 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Journal of Immunology Research

fig2

Figure 2

Figure 2: The BRD4 Inhibitor dBET57 Exerts Anticancer Effects by Targeting Superenhancer-Related Genes in Neuroblastoma 