Suppression of T-Cell Proliferation by Normal Density Granulocytes Led to CD183 Downregulation and Cytokine Inhibition in T-Cells

Figure 1

Inhibition of T-cell proliferation by NDGs and the effect of catalase, nor-NOHA, GAL, and CD11b on proliferation restoration. (a) Inhibition of T-cell proliferation by nonactivated NDGs (). Neutrophils suppress T-cell proliferation (), and addition of ROS inhibitor catalase protects T-cell proliferation (). The arginase inhibitor nor-NOHA did not have a protective effect and neither did TGF-β inhibitor GAL. Addition of anti-CD11b antibody protected T-cell proliferation (). (b) Inhibition of T-cell proliferation by fMLF activated NDGs (). fMLF activated NDGs inhibit T-cell proliferation (), and the proliferation was improved by catalase () and anti-CD11b (). nor-NOHA and GAL did not improve proliferation. Graphs indicate median with 95% CI, and statistical significance was tested using Wilcoxon matched pairs signed rank test.

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