lncRNA MALAT1 Promotes Diabetic Nephropathy Progression via miR-15b-5p/TLR4 Signaling Axis

Figure 3

MALAT1 binds with miR-15b-5p, and an miR-15b-5p inhibitor reverses HG-induced HK-2 cell activity via MALAT1. (a) The binding sites between MALAT1 and miR-15b-5p and MALAT1-Mt sequence in the binding sites. (b, c) MALAT1 luciferase activity in HK-2 cells cotransfected with MALAT1-Wt or MALAT1-Mt vector and miR-15b-5p mimics or mimics NC. versus the mimics NC group. (d) Anti-Ago2 RIP was carried out in HK-2 cells; then, the amount of MALAT1 or miR-15b-5p associated with Ago2 was detected by qRT-PCR. (e) Quantification of miR-15b-5p levels by qRT-PCR analysis in HK-2 cells transfected with si-MALAT1 under NG and HG conditions. (f, g) TLR4 mRNA by qRT-PCR analysis and protein levels by western blot analysis, (h) cell viability by CCK-8 assay, (i, j) cell apoptosis by TUNEL assay, and (k) TNF-α levels by ELISA analysis were evaluated in HG-induced HK-2 cells cotransfected with si-MALAT1 and miR-15b-5p inhibitor. versus the HG+si-MALAT1 group.

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