lncRNA MALAT1 Promotes Diabetic Nephropathy Progression via miR-15b-5p/TLR4 Signaling Axis

Figure 4

miR-15b-5p negatively regulates TLR4, and MALAT1 regulates TLR4 expression via miR-15b-5p. (a) TLR4 complementary sequences of wild-type (Wt) and mutant-type (Mt) TLR4 are shown with the miR-15b-5p sequence. (b) The inhibitory effect of miR-15b-5p on TLR4 3-UTR in HK-2 cells transfected with TLR4 3-UTR by Luciferase reporter assay. versus the mimics NC group. (c) Western blot analysis of TLR4 in HG-stimulated HK-2 cells transfected with miR-15b-5p mimics or mimics NC. (d) Cell viability by CCK-8 assay, (e, f) cell apoptosis by TUNEL assay, and (g) TNF-α levels by ELISA analysis were examined in HG-induced HK-2 cells cotransfected with si-MALAT1 and vector TLR4. versus HG+si-MALAT1+vector TLR4.

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