Transmembrane Protein ANTXR1 Regulates γ-Globin Expression by Targeting the Wnt/β-Catenin Signaling Pathway

Figure 1

Expression of ANTXR1, γ-globin, and β-catenin during erythroid differentiation. (a) Western blot analysis was performed to detect the expression levels of ANTXR1, γ-globin, and β-catenin in K562 cells and HUDEP-2 cells at days 0, 4, and 7 of erythroid differentiation. (b) Western blot analysis for the expression of ANTXR1, γ-globin, and β-catenin in umbilical cord blood CD34⁺ cells during erythroid differentiation at days 8, 11, 13, 14, 15, and 16. (c) Western blot analysis for the expression of ANTXR1, γ-globin, and β-catenin in adult peripheral blood CD34⁺ cells during erythroid differentiation at days 8, 11, 13, 14, 15, and 16. (d) Protein quantification of the western blot in HUDEP-2 cells ANTXR1, γ-globin, and β-catenin at days 0, 4, and 7. (e) Quantification of western blot results of ANTXR1, γ-globin, and β-catenin in umbilical cord blood CD34⁺ cells at days 8, 11, 13, 14, 15, and 16. (f) Quantification of western blot results of ANTXR1, γ-globin, and β-catenin in adult peripheral blood CD34⁺ cells at days 8, 11, 13, 14, 15, and 16. These experiments . The error bar represents the SD. and .

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