Transmembrane Protein ANTXR1 Regulates γ-Globin Expression by Targeting the Wnt/β-Catenin Signaling Pathway

Figure 6

Overexpression of ANTXR1 promoted the combination of c-Jun and SOX6. (a) ChIP-qPCR assays were performed using K562 cells expressing ANTXR1, while an empty vector served as a control. The immunoprecipitated DNA of SOX6, EIF2AK, BGLT3, and ZBTB7A 4 binding site to c-Jun, as well as input DNA, were used as templates for quantitative fluorescence PCR. The enrichments were quantified by ChIP-qPCR and normalized by comparison to input DNA (% input). (b) After overexpression of c-Jun in K562 cells, the protein levels of c-Jun and SOX6 were measured by western blotting. (c, d) Quantification of the western blot. The experiments . The error bar represents the SD. .

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