Aerosol Inhalation of Heat-Killed Clostridium butyricum CGMCC0313-1 Alleviates Allergic Airway Inflammation in Mice

Figure 4

The effect of aerosol inhalation of HKCB on the NF-κB/NLRP3 inflammasome signaling pathway in the lung tissues of allergic mice. (a) Expression levels of NF-κB p65 and NLRP3 in lung tissues, as examined by immunohistochemistry staining (magnification 200x). (b, c) Expression scores of NF-κB p65 and NLRP3 in lung tissues. (d) Expression levels of NF-κB p65, NLRP3, ASC, pro-caspase1, caspase1 p20, and mature-IL-1β in lung tissues were examined using western blotting. (e–j) Relative expression levels of NF-κB p65, NLRP3, ASC, pro-caspase1, caspase1 p20, and mature-IL-1β in lung tissues. and versus the NC group; ^#, ^##, and ^### versus the OVA group. a.i.: aerosol inhalation; CB: Clostridium butyricum; HKCB: heat-killed CB.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)