The Regulation between CD4⁺CXCR5⁺ Follicular Helper T (Tfh) Cells and CD19⁺CD24^hiCD38^hi Regulatory B (Breg) Cells in Gastric Cancer

Figure 1

B cell facilitate Tfh cell differentiation and CXCL13 secretion in vitro. Purchased T cells and B cells were obtained from venous blood of healthy people. Tfh cells were stimulated with cytokines and with or without B cell cocultured (a). Tfh cells’ gate (b) and its percentage variation in the coculture groups (c). Breg cells’ gate (d) and its percentage variation in the coculture groups (e). The level of IL-10 (f), CXCL13 (g), and IL-21 (h) in the coculture groups. Tfh: follicular help T cells; Breg: regulatory B cells; IL-10: interleukin-10; CXCL13: C-X-C motif chemokine ligand 13; IL-21: interleukin-21; : <0.05; : <0.01; ns: no significance.

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