Blockade of NLRP3/Caspase-1/IL-1β Regulated Th17/Treg Immune Imbalance and Attenuated the Neutrophilic Airway Inflammation in an Ovalbumin-Induced Murine Model of Asthma

Figure 3

NLRP3/caspase-1 inhibitor downregulated inflammasome activation and downstream factors. (a, b) Protein expression of NLRP3 (117 KD) and IL-1β (35 KD) in lung homogenates was detected by western blot, and densitometric analysis was performed. (c) The concentration of IL-1β and IL-18 in BALF was quantified by ELISA. (d) mRNA expression of Nlrp3, caspase-1, IL-1β, and IL-18 in lung homogenates was detected by real-time PCR. Data are expressed as . . Compared to the control group, , , and ; compared to the neutrophilic asthma group, ^#, ^##, and ^###. NLRP3: nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3; BALF: bronchoalveolar lavage fluid; ELISA: enzyme-linked immunosorbent assay; PCR: polymerase chain reaction.

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