Blockade of NLRP3/Caspase-1/IL-1β Regulated Th17/Treg Immune Imbalance and Attenuated the Neutrophilic Airway Inflammation in an Ovalbumin-Induced Murine Model of Asthma

Figure 5

Inhibition of the NLRP3/caspase-1/IL-1β pathway upregulated ovalbumin-induced Treg cell response. (a, b) Splenocytes were derived from mice and fixed and permeabilized, and cells were incubated with extracellular and intracellular antibodies. Stained cells were run on the CytoFLEX Flow Analyzer and analyzed with CytExpert software. (c) The concentration of IL-10 in BALF was quantified by ELISA. (d) mRNA expression of Foxp3 in lung homogenates was detected by real-time PCR. Data are expressed as . . Compared to the control group, , , and ; compared to the neutrophilic asthma group, ^#, ^##, and ^###. NLRP3: nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3; BALF; bronchoalveolar lavage fluid; ELISA: enzyme-linked immunosorbent assay; Foxp3: fork head box p3; PCR: polymerase chain reaction.

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