Long Noncoding RNA TUG1 Inhibits Tumor Progression through Regulating Siglec-15-Related Anti-Immune Activity in Hepatocellular Carcinoma

Figure 1

TUG1 is elevated in HCC cells and controls the Siglec-15 expression. (a) Schematic diagram of lncRNAs screen by bioinformation. (b) TUG1 expression in HCC patients from TCGA database. (c) Coexpression relationship between TUG1 and Siglec-15 in clinical data from TCGA database. (d) TUG1 expression, (e) Siglec-15 mRNA expression, and (f, g) protein expression in different HCC cell lines (HepG2, QGY-7701, Bel-7407, SK-Hep-1, Hep3B, and HUH7), human normal hepatic cell line LO-2, and primary human hepatocytes HH. (h, i) Siglec-15 mRNA expression and (j, k) protein expression in HUH7 and Hep3B cells treated with TUG1 overexpression or knockdown. (l, m) Relative luciferase activity of Jurkat-RGA in HUH7 and Hep3B cells treated with TUG1 overexpression or knockdown. (n, o) T cell-induced cytotoxicity cocultured with HUH7 or Hep3B cells treated with TUG1 overexpression or knockdown. (p, q) IFN-γ and (r, s) IL-2 secreted by T cells from (n, o). The data are presented as the , experiments in (d–s). , , . S15: Siglec-15.

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