Research Article
Long Noncoding RNA TUG1 Inhibits Tumor Progression through Regulating Siglec-15-Related Anti-Immune Activity in Hepatocellular Carcinoma
| | No. | Target sequence
21nt target + 2nt overhang | RNA oligo sequences
21nt guide (5→3)
21nt passenger (5→3) |
| | 3453 bp-3713 bp | | si-TUG1-1 | GGCCTGAATCCTGCTACAACTAT | AGUUGUAGCAGGAUUCAGGCC | | CCUGAAUCCUGCUACAACUAU | | si-TUG1-2 | TGCTACAACTATCTTCCTTTACC | UAAAGGAAGAUAGUUGUAGCA | | CUACAACUAUCUUCCUUUACC | | si-TUG1-3 | TACAACTATCTTCCTTTACCACC | UGGUAAAGGAAGAUAGUUGUA | | CAACUAUCUUCCUUUACCACC | | si-TUG1-4 | TTCCTTACAACACCTTGAACTCT | AGUUCAAGGUGUUGUAAGGAA | | CCUUACAACACCUUGAACUCU |
| | 5514 bp-5820 bp | | si-TUG1-5 | GCCTTGACTTGCTTGTAAGATGA | AUCUUACAAGCAAGUCAAGGC | | CUUGACUUGCUUGUAAGAUGA | | si-TUG1-6 | ACGACTTGATTACCAAAGAAAGT | UUUCUUUGGUAAUCAAGUCGU | | GACUUGAUUACCAAAGAAAGU | | si-TUG1-7 | GACTTGATTACCAAAGAAAGTAG | ACUUUCUUUGGUAAUCAAGUC | | CUUGAUUACCAAAGAAAGUAG | | si-TUG1-8 | TAGCATAGACTCCTAAACAGAAC | UCUGUUUAGGAGUCUAUGCUA | | GCAUAGACUCCUAAACAGAAC | | si-TUG1-9 | AGCATAGACTCCTAAACAGAACC | UUCUGUUUAGGAGUCUAUGCU | | CAUAGACUCCUAAACAGAACC | | si-TUG1-10 | CTGTAAGATCAGAAAACTGTATC | UACAGUUUUCUGAUCUUACAG | | GUAAGAUCAGAAAACUGUAUC |
| | 6941 bp-7107 bp | | si-TUG1-11 | CTGGACTTTTCAGTTATGTGAAC | UCACAUAACUGAAAAGUCCAG | | GGACUUUUCAGUUAUGUGAAC | | si-TUG1-12 | TGGACTTTTCAGTTATGTGAACC | UUCACAUAACUGAAAAGUCCA | | GACUUUUCAGUUAUGUGAACC | | si-TUG1-13 | TTCAGTTATGTGAACCAATAAAT | UUAUUGGUUCACAUAACUGAA | | CAGUUAUGUGAACCAAUAAAU | | si-TUG1-14 | CAGTTATGTGAACCAATAAATAC | AUUUAUUGGUUCACAUAACUG | | GUUAUGUGAACCAAUAAAUAC | | si-TUG1-15 | AACCAATAAATACCCTTTTTTGC | AAAAAAGGGUAUUUAUUGGUU | | CCAAUAAAUACCCUUUUUUGC |
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