Antibodies Recognizing Yersinia enterocolitica Lipopolysaccharides of Various Chemotypes in Synovial Fluids From Patients With Juvenile Idiopathic Arthritis

Figure 2

In vitro processing of Yersinia LPS. PMA differentiated THP-1 cells were fed with heat-killed bacteria of various chemotypes. After 2 or 5 days of cultivation, cells were fixed with PFA and incubated with human serum as a source of MBL. The cell-surface bound MBL was detected with an MBL-specific monoclonal antibody and Alexa Fluor 488 labelled secondary antibody.