Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages’ Function but Does Not Trigger the Adaptive T Cell Immune Response

Figure 2

Monoclonal α-CLEC5A Ab triggers a time-dependent cytokine secretion from U937 WT but not from U937 CLEC5A KO cell line. (a) PMA-differentiated WT U937 cells were transferred on culture plates previously coated with 20 μg/mL of α-CLEC5A Ab (grey symbols) or isotype ctrl (black symbols). Cell culture supernatants harvested after 16 h (upper panel), 24 h (middle panel), and 48 h (bottom panel) were tested for the indicated cytokines with MSD or ELISA kits. (b) PMA-differentiated U937 KO cells were incubated 48 h on culture plates previously coated with 20 μg/mL of α-CLEC5A Ab (grey symbols) or isotype ctrl (black symbols). Cell culture supernatants were tested for different cytokines with MSD or ELISA kits. Values represent the mean and SD calculated from three technical replicates/condition; , , and .

(a)

(b)