Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages’ Function but Does Not Trigger the Adaptive T Cell Immune Response

Figure 3

CLEC5A receptor is highly expressed on human myeloid cells. PBMC isolated from healthy donors were stained at ⁵ cells in 100 μL/well with a FACS staining solution containing selected antibodies for myeloid and lymphoid cell surface markers: CLEC5A (1 : 40), CD14 (1 : 80), CD16 (1 : 10), CD11c (1 : 20), CD4 (1 : 160), and CD8 (1 : 20). CLEC5A expression was measured by flow cytometry. (a) CLEC5A expression (grey line) vs negative ctrl (grey shadow) on selected immune cell populations: myeloid cells (upper panel) and lymphoid cells (lower panel). Shown are exemplary histograms from one representative donor. (b) Frequency of CLEC5A-expressing cells (shown as percentage of CLEC5A-positive cells) in selected immune cell populations from 3 individual donors. (c) Median fluorescence intensity (MFI) of CLEC5A expression in selected immune cell populations from 3 individual donors. Shown is one representative experiment from two independent measurements (six donors, three technical replicates per donor) with similar outcome.

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