Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages’ Function but Does Not Trigger the Adaptive T Cell Immune Response

Figure 7

CLEC5A receptor agonist modulates the surface expression of other myeloid cell-specific receptors. M0 MdM generated from same donors as in Figures 5–6 were incubated for 72 h on culture plates coated either with the α-CLEC5A Ab or with the isotype ctrl (10 μg/mL) and then FACS stained with directly labelled monoclonal Abs against different membrane receptors. For FACS staining, monoclonal antibodies were diluted 1 : 40 in 100 μL FACS buffer. Graphs depict the expression of each marker shown as MFI for each donor tested, while histograms illustrate an exemplary FACS fluorescence in one representative donor (grey line: MdM incubated with α-CLEC5A Ab; grey shadow: MdM incubated with isotype ctrl). Values represent the mean and SD from three technical replicates per each donor. Shown is the summary result from two independent experiments with donors; , , and .

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