Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages’ Function but Does Not Trigger the Adaptive T Cell Immune Response

Figure 8

CLEC5A receptor activation on MdM does not support autologous T cell priming. MdM generated from monocytes in the presence of M-CSF (a) or GM-CSF (b) were cocultured for 48 h with pan T cells that were purified from same donors and preactivated with 0.1 μg/mL a-CD3 Ab. T cell-derived IL-2 secretion was measured by ELISA in cell culture supernatants. Proliferation of CD4+ (left) and CD8+ T cells (right) cocultured for 5 days with M-CSF (c) or GM-CSF (d) macrophages in the presence of 0.1 μg/mL α-CD3 Ab was measured by flow cytometry. The cells were stained in 100 μL FACS buffer containing 1 : 20 diluted FACS antibodies against CD4 and CD8 antigens. Values represent the mean and SD from three technical replicates per donor. Shown is the summary result of two independent experiments with six donors (a and b) and one experiment with three donors (c and d); , , and .

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