Identification of Hub Biomarkers and Immune and Inflammation Pathways Contributing to Kawasaki Disease Progression with RT-qPCR Verification

Figure 3

Detection of differentially expressed genes and selection of hub genes: (a) a volcano plot shows DEGs that compare healthy control tissues with those affected by KD; (b) a Venn diagram depicting the intersection of DEGs and the blue module. Thirty-six DEGs were identified as intersecting; (c) comparing LASSO regression with partial likelihood deviance while altering log (l) in 10-fold cross-validations. Vertical dashed lines mark the ideal (lambda.min) values defined by the minimal criterion (1-SE criterion); (d) LASSO coefficient profiles were produced from 10-fold cross-validation for the six hub genes. Abbreviations: DEGs, differentially expressed genes; KD, Kawasaki disease; LASSO, least absolute shrinkage and selection operator.

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