Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow

Figure 1

Microfluidics assay, TLR ligands, and switching experiment. Collagen surfaces were patterned on glass slides using single channel patterning devices, then PPACK WB was collected from healthy donors, platelets were labeled with anti-CD61, and actual flow assay were carried out in 8-channel devices (a). TLR ligands and their corresponding TLRs are listed in a table (b). For switching experiment, setting up procedures are same as the flow assay, experiments were running with control condition WB for the first 90s, then switch to the WB with TLR ligands. Monolayer of collagen adherent platelet was formed for the first 90s, then platelets from WB with TLR ligands will aggregate on the first platelet layer.

(a)

(b)

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