Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow

Figure 6

Pam3CSK4, MALP-2, and Imquimod can inhibit PS exposure under flow. High CTI WB with HBS (control) or different TLR ligands was perfused over collagen at 100 s^-1 for 720 s. CD61, fluorescence fibrinogen fluorophores, and Annexin V fluorophores were added to label for platelets, fibrin, and PS exposure, respectively. Platelet FI (a, d, and g), Fibrin FI (b, e, and h), and Annexin V FI (c, f, and i) were measured throughout the course of the experiments.