miR-1 Inhibits the Ferroptosis of Chondrocyte by Targeting CX43 and Alleviates Osteoarthritis Progression

Figure 2

miR-1 played an essential role in regulating chondrocyte functions. (a) Cell proliferation of chondrocytes detected by EdU assay. (b) Quantitative analysis of the EdU assay. (c) CCK-8 assay used to detect the proliferation ability of chondrocytes. (d) Representative images showing the migration ability of chondrocytes by scratch wound healing assay. (e) Representative images showing migrated chondrocytes by transwell assay. (f) Quantitative analysis of scratch wound healing assay. (g) Quantitative analysis of the migrated chondrocytes in transwell assay. (h) Representative images of flow cytometry showing apoptosis rate of chondrocytes using Annexin V/FITC/PI double-staining. (i) Quantitative analysis of the apoptosis rate of chondrocytes. (j) The protein expression levels of aggrecan, Col2, Sox9, MMP13, BCL-2, and cleaved caspase-3 in chondrocytes detected by Western blot assay. Scale bar = 200 µm. Data were presented as the mean ± SD of at least three replicates. and .

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